Comparison of the Diagnostic Values of Dynamic Enhanced Magnetic Resonance Imaging,Digital Breast Tomosynthesis,and Digital Mammography for Early Breast Cancer / 中国医学科学院学报

Objective To compare the values of dynamic enhanced magnetic resonance imaging(DCE-MRI),digital breast tomosynthesis(DBT),and digital mammography(DM)in the early detection and diagnosis of breast cancer.Methods We retrospectively analyzed the clinical and imaging data of 65 cases with early breast cancer confirmed by surgical pathology from June 2017 to December 2018.All patients underwent breast DCE-MRI,DM and DBT before surgery.The receiver operating characteristic(ROC)curves were drawn,with the pathological results as the gold standard,to evaluate the diagnostic performance of different examination methods.The areas under ROC curves(AUCs)were compared using test.The differences among DCE-MRI,DBT and DM in detecting early breast cancer were compared using chi-square test in terms of positive rates,accuracy,sensitivity,and specificity.Pearson correlation analysis was performed to assess the accuracy of these imaging methods in detecting the size of early breast cancer.Results The AUCs of DCE-MRI,DBT,and DM based on the BI-RADS classification for early diagnosis of breast cancer were 0.910,0.832,and 0.700,respectively(=2.132,=0.001);the sensitivity of DCE-MRI,DBT,and DM for early breast cancer was 92.3%,70.8%,and 52.5%,the specificity was 65.0%,85.0%,and 79.3%,and the accuracy was 83.1%,70.8%,and 50.8%,indicating that DCE-MRI(=15.330,=0.0001) and DBT(=5.450,=0.020) had significantly higher diagnostic accuracy than DM.The measurement results of DM,DBT,and DCE-MRI were positively correlated with the pathological measurements(=0.781,=0.847,=0.946;all <0.01). Conclusions DCE-MRI and DBT have higher positive rates and accuracies than DM in detecting early breast cancer.Medical institutions where DCE-MRI is still not available can use DBT to improve the early detection of breast cancer.

Expression of vascular endothelial growth factor in deep second-degree scald wounds in rats / 中国医学科学院学报

<p><b>OBJECTIVE</b>To observe the expression of vascular endothelial growth factor(VEGF)in deep second-degree scald wounds,with an attempt to further explore the role of VEGF in burn wound healing.</p><p><b>METHODS</b>Totally 36 adult Wistar rats were randomized into two groups: the scald group(30 rats)and the control group(6 rats). In the scald group,rat models of deep second-degree scald wounds were established. Full-thickness tissues of the wounds were collected respectively 1,3,7,14,and 21 days after the modeling. The expressions of the VEGF mRNA and protein were detected with real-time quantitative PCR and Western blot,respectively. In the control group,the same procedures were performed but without modeling.</p><p><b>RESULTS</b>Compared with the control group,the expressions of VEGF mRNA and proteins were significantly higher in the scald group(P<0.05). The expression levels reached the peak on day 1,gradually decreased on day 3,reached the lowest points on day 14,but increased again on day 21.</p><p><b>CONCLUSIONS</b>VEGF is involved in the healing of scald burns. The expression of VEGF during the wound healing is closely correlated with the wound angiogenesis.</p>

Expression of aquaporin-1 in myocardium of rats in the early stage after scald and its relationship with myocardial edema / 中华烧伤杂志

<p><b>OBJECTIVE</b>To investigate the changes in aquaporin-1 (AQP-1) expression in myocardium of scalded rats in early stage of a burn injury, and to analyze its relationship with myocardial edema.</p><p><b>METHODS</b>Thirty-six healthy Wistar rats were divided into normal control (n = 6, without scald) and scald (n = 30) groups according to the random number table. Rats in scald group were inflicted with 30%TBSA full-thickness scald on the back, and intraperitoneally injected with Ringer's solution for antishock treatment. Myocardium tissue from left ventricle and serum specimen in rats of scald group were collected at post scald hours (PSH) 2, 8, 12, 24, and 48 (with 6 rats at each time point). Myocardial water ratio was determined by dry-wet weight method. The distribution of AQP-1 protein in myocardium was observed with immunohistochemical staining. The expression of myocardial AQP-1 mRNA was assessed with quantitative real-time PCR. The serum content of cardiac troponin-I (cTnI) was determined with ELISA. The rats in normal control group were detected with above-mentioned method. Data were processed with one way analysis of variance and LSD test. Correlation analysis was performed between AQP-1 mRNA and myocardial water ratio, AQP-1 mRNA and the serum content of cTnI in scald group at each time point.</p><p><b>RESULTS</b>Compared with that in normal control group, the myocardial water ratio in scald group was markedly increased during PSH 8-48 (P values all below 0.01), and it peaked at PSH 12 [(80.79 ± 0.12)%]. In both groups, AQP-1 was mainly expressed in endothelial cells of capillaries and pericellular membrane of myocardial cells. The expression of AQP-1 in scald group was markedly increased from PSH 2 to PSH 48. The expression of myocardial AQP-1 mRNA in scald group was markedly higher from PSH 2 to PSH 48 than that in normal control group (P values all below 0.01), and it peaked at PSH 12 [(6.2 ± 0.7)%]. The serum content of cTnI in scald group was obviously higher from PSH 2 to PSH 48 than that in normal control group (P values all below 0.01), and it peaked at PSH 12 [(5.83 ± 0.51) µg/L]. There were statistically positive correlations between AQP-1 mRNA expression and myocardial water ratio (r = 0.849, P < 0.01), AQP-1 mRNA expression and the serum content of cTnI (r = 0.973, P < 0.01) in scald group.</p><p><b>CONCLUSIONS</b>AQP-1 may play a key role in the development of myocardial edema in rats with scald.</p>

Treatment of a patient with massive unhealed wound accompanied by wound sepsis in late stage after burn injury / 中华烧伤杂志

This article analyzed the medical records of a patient with 90% TBSA unhealed wound accompanied with wound sepsis 50 days post burn (PBD) and to discuss the ideal strategies of treatment for such patients in such condition. This was a 24-year-old male patient suffering from flame burn with 95% TBSA wound and severe inhalation injury. Meek skin grafting with autologous scalp was performed once to the thoracic and abdominal regions; intermingled skin grafting of autologous scalp microskin and large sheet of allograft was performed twice to the limbs within PBD 31. The patient was transferred to our hospital on PBD 50 with 90% TBSA wound unhealed, leaving a vast amount of necrotic tissue and allografts. Furthermore, he was complicated by sepsis, pulmonary infection, and gastric ulcer. Debridement and allogenic skin grafting were performed on the first day after hospitalization. When the condition of wounds was improved, transplantation of a large sheet of allogenic skin with inlaid small pieces of autologous skin, intermingled skin grafting of autologous and allogenic skin, and small pieces of autologous skin grafting were performed. Because of the shortage of donor area, the exposed wounds were temporarily covered with allogeneic skin. Epidermal growth factor was used to promote the healing of autologous skin donor site and deep partial-thickness burn wound. Autologous skin grafting was performed whenever source of healthy skin was available. Systemic use of effective antibiotics, nutritional support and therapy, and other comprehensive measures also contributed to the success of treatment of this patient suffering from wound sepsis. The patient was cured and discharged on PBD 145.

Effects of exendin-4 on rat cardiomyocyte apoptosis early after severe scald injury / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the effect of exendin-4 on cardiomyocyte apoptosis in the early stage after scald injury in rats and explore the mechanisms.</p><p><b>METHODS</b>Fifty-four healthy adult SD rats were randomly divided into normal control group (n=6), scald group (n=24) and scald with exendin-4 treatment group (n=24). In the latter two groups, the rats were subjected to 30% TBSA full-thickness scald burns on the back, and Parkland formula was used for determining the resuscitation fluid volume. In exendin-4 treatment group, the rats received intraperitoneal injection of 5 µg/kg exendin-4 after the scald. Apoptosis of the cardiomyocytes from the left ventricle was determined by TUNEL assay and the activity of caspase-3 in the myocardium was assessed.</p><p><b>RESULTS</b>In the scald group, the apoptotic index of the cardiomyocytes was increased at 6 h post-burn, reaching the peak level at 12 h, and maintained a significantly higher level than that in the normal control at 48 h (P<0.05). Myocardial caspase-3 activity in the scald group was increased at 6 h post-burn and reached the peak at 12 h, still maintaining a high levels at 24 h (P<0.05). In exendin-4 treatment group, the apoptotic index of the cardiomyocytes was significantly lower than that in the scald group at 6, 12, 24 and 48 h post-burn (P<0.05), and so was the caspase-3 activity at 6, 12 and 48 h (P<0.05). A significant positive correlation was found between the apoptotic index of the cardiomyocytes and myocardial caspase-3 activity in the rats (P<0.05).</p><p><b>CONCLUSION</b>Exdendin-4 can inhibit rat cardiomyocyte apoptosis early after scald injury possibly by suppressing caspase-3 activity in the myocardium.</p>

Changes of aquaporin-1 expression in rat myocardium after severe burn / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the changes in the myocardial expression of aquaporin-1 (AQP1) protein and its association with myocardial edema in rats with severe burns.</p><p><b>METHODS</b>Forty-eight healthy adult Wistar rats were randomly divided into normal control group (n=6) and burn injury group with third degree burn of 30% total body surface area, and the latter group was further divided into 2, 4, 8, 12, 24, 48 and 72 h groups. The changes of myocardial water content were investigated by dry-wet weight methods. Enzyme-linked immunosorbent assay was used to detect the changes in AQP1 expression at different time points after sever burns.</p><p><b>RESULTS</b>The myocardial water content and AQP1 expression increased significantly 2 h after the burn injury, reaching the peak levels at 12 h and remaining higher than the normal level at 48 h. A significant positive correlation was found between myocardial water content and AQP1 expression in the rats (r=0.868, P<0.01).</p><p><b>CONCLUSION</b>The severity of myocardial edema after severe burn is correlated to the expression level of AQPl protein, suggesting the important role of AQPl protein in pathological progression of myocardial edema.</p>

Effect of early enteral feeding on the expression of aquaporin 1 in edematous small intestinal tissues of rats after severe burn / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the changes in the expression of aquaporin 1 (AQP-1) in edematous small intestinal tissues of rats after severe burn and the effect of early enteral feeding on its expression.</p><p><b>METHODS</b>Ninety normal adult Wistar rats were randomly divided into normal control group (n=6), burn model group (n=42, with 30% TBSA III degrees) and early feeding group (n=42). Dry weight method, ELSIA and immunohistochemistry were used to observe and detect the water content and expression of AQP-1 in the intestinal tissue at 1, 4, 8, 12, 24, 48, and 72 h after the burns.</p><p><b>RESULTS</b>In the burn model group, the water content in the intestinal tissue increased at 4 h after the injury, reaching the peak level at 48 h; AQP-1 expression decreased at 8 h after severe burn and reached the lowest level at 48 h. AQP-1 expression level showed a significant inverse correlation to the water content (P<0.01). Compared with the burn model group, the rats in the early feeding group showed increased AQP-l expression and lessened edema in the small intestines, also demonstrating an inverse correlation between water content and AQP-l expression (P<0.01).</p><p><b>CONCLUSION</b>Intestinal AQP-1 expression gradually decreased and edema worsened in rats early after severe burn, reaching the lowest or the peak levels 48 h after the injury with an inverse correlation between them. Early enteral feeding can increase the expression of AQP-l in the small intestine to ameliorate the intestinal edema in rats with severe burn injury.</p>

Effect of nuclear factor-kappaB activation on expression of proinflammatory cytokines in rat lung tissues in early stage of burn injury / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effect of nuclear factor-kappaB (NF-kappaB) activation on the expression of proinflammatory cytokines in the lung tissues of rats with early-stage burn injury.</p><p><b>METHODS</b>Wistar rats were randomly divided into 3 groups, namely the normal control, burn, burn and PDTC treatment groups, and in the latter two groups, the rats were subjected to 35% TBSA full-thickness burns. Activation of pulmonary NF-kappaB at 1, 3, 6, 12, and 24 postburn hour (PBH) was tested by electrophoretic mobility shift assay , and the expressions of pulmonary tumor necrosis factor alpha (TNF alpha) and interleukin-8 (IL-8) mRNAs at 3, 6, 12, and 24 h were detected by RT-PCR.</p><p><b>RESULTS</b>Compared to that of the control group, activity of pulmonary NF-kappaB in burned rats was markedly increased within 1 PBH and kept increasing till 24 h. Expressions of pulmonary TNF alpha and IL-8 mRNAs increased gradually, reaching the peak level at 6 PBH, and PDTC could effectively inhibit pulmonary NF-kappaB activation and expression of the pulmonary cytokines induced by the burn injury.</p><p><b>CONCLUSION</b>Severe burn injury may activate pulmonary NF-kappaB, which ultimately leads to secretion of cytokines in the lung tissues.</p>

Sequential management of residual wounds in burn patients / 中华烧伤杂志

<p><b>OBJECTIVE</b>To seek a sequential method for the management of residual wounds in burn patients.</p><p><b>METHODS</b>Three chronic residual wounds on each of 25 burn patients were either covered with vaseline gauze (A group), human tissue-engineered active skin (Active Skin, B group) or Active Skin after rinsing with fluid containing oxygen and vacuum assisted drainage ( C group) on wounds. The contents of (TNF)a in granulation tissue were assayed by enzyme linked immunosorbent assay (ELISA). Expression of metalloproteinase-13 (MMP-13) mRNA in granulation tissue was determined with reverse transcription polymerase chain reaction (RT-PCR). Moreover, quantity of wound bacteria in the wounds and wound healing rate were determined with usual method.</p><p><b>RESULTS</b>The quantities of wound bacteria in C group on 3,6,9, 12 post-treatment day( PTD) were (5.30 +/- 1.60), (1.30 +/-0.80) , (1.70 +/- 0. 60)and (0.60 +/-0. 10)clone formation unit/ml( CFU/ml) , respectively, which were obviously lower than those in A and B groups. The contents of TNFa and expression of metalloproteinase-13 (MMP-13) mRNA in granulation tissue in C group on 6 PTD were [ (0. 650 +/- 0. 040) ng/mg and 0. 210 +/- 0. 010,] ,respectively, and they were evidently lower than those in A group [(1.550 +/-0. 370)ng/mg,1. 040 +/- 0. 050, P <0.01] and B group (0. 810 +/- 0.080) ng/mg, 0.640 +/- 0.030, P <0.01]. Meanwhile, the contents of (TNF)a and expression of MMP-13 mRNA in B group were also obviously lower than those in A group. The wound healing ratio in C group on 15 and 30 PTD were markedly higher than those in A or B group ( P <0.01).</p><p><b>CONCLUSION</b>Covering the residual burn wounds with Active Skin after rinsing with fluid containing oxygen followed by vacuum assisted drainage can improve repairing of residual burn wounds.</p>

Effect of escharectomy on rats'pulmonary NF-?B activation in early stage of burn injury / 中华急诊医学杂志

Objective To investigate the effect of escharectomy on rats' pulmonary NF-?B activation and the expression of pulmonary proinflammatory cytokines in early stage of burn injury.Method Wistar rats were randomly divided into three groups:group A(control group),group B(postburn without escharectomy),group C(escharectomy at early stage of burn injury).Thermal-injuried rats underwent 35% TBSA full-thickness burns. Activation of pulmonary NF-?B at 12 hours and 24 hours postburn was tested by electrophoretic mobility shift assay (EMSA),and at the same time expressions of pulmonary TNF-?mRNA were measured by reverse transcription polymerase chain reaction(RT-PCR)and release of pulmonary TNF-?were assayed by enzyme-linked immunosorbent assay(ELISA).Results Compared with control group,activity of pulmonary NF-?B in group B was markedly increased,reached(19.56?1.36)?10~4 A at 12 hours and(15.23?1.94)?10~4 A at 24 hours,which was higher than that in group A[(4.36?0.38)?10~4 A,P

Influence of sea water immersion on inflammation and healing of the wounds in scalded rats / 中华烧伤杂志

<p><b>OBJECTIVE</b>To investigate the influence of sea water immersion on inflammation and healing of the wounds in scalded rats.</p><p><b>METHODS</b>One hundred and forty-four male Wistar rats with 10% TBSA superficial partial-thickness scald were randomly divided into A (n=72, with scald) and B (n=72, with seawater immersion for 4hrs immediately after scald) groups. The serum contents of K+, Na+, Cl- were determined at 0 post-scald hour (PSH), 6PSH, 12PSH and 24 PSH with electrocyte analysis apparatus, and the changes in serum interleukin-6 (IL-6) and tumor necrosis factor (TNF-alpha) levels were determined at 0 PSH, 6PSH and 12 PSH with enzyme-linked immunosorbent assay (ELISA). The histopathological changes in the rats of the two groups were observed, and wound healing time was respectively calculated.</p><p><b>RESULTS</b>The serum contents of K+, Na+, Cl- in B group were obviously higher than those in A group. And the serum content of TNF-alpha and IL-6 in B group at 6 PSH [(140 +/- 22) ng/L, (160 +/- 41) ng/L] were significantly higher than those before scald [(29 +/- 15) ng/L, (62 +/- 17)] ng/L and in A group [(120 +/- 12) ng/L, (124 +/- 22) ng/L, ( P < 0.05)]. Compared with A group, re-epithelization of the wound differentiation in all layers of epidermis were delayed in B group, with more severe wound swelling, exudation, and topical inflammatory response. The wound healing time in B group was (16.3 +/- 1.6) d, which was obviously longer than that in A group [(14.1 +/- 1.8) d, P < 0.05)].</p><p><b>CONCLUSION</b>Sea water immersion combined with scald injury can aggravate the inflammatory response of the wound and delay the wound healing process.</p>

The role of nuclear factor-kappaB in the burn serum-induced expression of intercellular adhesion molecules-1 in endothelial cells / 中华烧伤杂志

<p><b>OBJECTIVE</b>To explore the role of nuclear factor-kappaB (NF-kappaB) in the burn serum induced expression of intercellular adhesion molecules-1 (ICAM-1) in endothelial cells.</p><p><b>METHODS</b>Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and divided into control (with normal serum stimulation), burn serum (B, with burn serum stimulation) and PDTC (with burn serum and PDTC stimulation) groups. The NF-kappaB activity in endothelial cells was determined with electrophoretic mobility shift assay (EMSA) at 0.5, 1.0, 2.0, 4.0 post-stimulation hour (PSH). The expression of ICAM-1 at 3.0, 6.0, 12. 0, 24.0 PSH was detected by flow cytometry.</p><p><b>RESULTS</b>The NF-kappaB activity in endothelial cells in burn serum group and PDTC group were markedly higher than that in control group (P <0.01), and it reached the peak at 1.0 PSH [(21.03 +/- 4.87), (7.44 +/- 0.60) x 10(4) A], respectively, then gradually decreased. But it was obviously lower in PDTC group than that in burn serum group ( P <0. 01 ). The expression of ICAM-1 was gradually increased in both burn serum group and PDTC group, reaching the peak level at 12.0 PSH [(327 +/- 37), (142 +/- 31) mean fluorescence intensity], respectively, which were significantly higher than that in control group (P <0.01). But it was evidently lower in PDTC group than that in burn serum group at 12.0 and 24.0 PSH (P <0.01).</p><p><b>CONCLUSION</b>Burn serum can initiate the synthesis and release of adhesion molecules in endothelial cells through activation of NF-kappaB, indicating that NF-kappaB plays an important role in the process of burn serum induced endothelial secretion of adhesion molecules.</p>

Effect of NF-kappaB activation on the early expression of tumor necrosis factor alpha and myocardial dysfunction in burned rats / 中华烧伤杂志

<p><b>OBJECTIVE</b>To investigate the effect of NF-kappaB activation on the early expression of proinflammatory cytokines in myocardium and early myocardial dysfunction in burn rats.</p><p><b>METHODS</b>One hundred and seventy Wistar rats were enrolled in the study and randomly divided into three groups, i. e. control( C, n = 20, with isotonic saline solution) , burn ( B, n = 90, with isotonic solution after burns) and pyrrolidine dithiocarbamate (PDTC, n =60, with isotonic saline and 250 mg/kg PDTC after burns) groups. The rats in B and PDTC groups were inflicted with 35% TBSA full-thickness burns on the back. The activity of myocardial NF-kappaB was determined by electrophoretic mobility shift assay at 1 , 3, 6, 12,24 postburn hours (PBH), with expression of integral absorbance ( A ) value . The expression of myocardial tumor necrosis factor alpha ( TNF-alpha) mRNA was assessed by reverse transcription polymerase chain reaction( RT-PCR) and in situ hybridization at 6, 12 PBH, with expression ofA value. The left ventricular systolic pressure( LVSP) , the left ventricular end diastolic pressure (LVEDP) ,the maximum rate of rise of left ventricular pressure ( +/- dp/dt max) were also observed at 3, 6, 12,24 PBH. RESULTS The activity of myocardial NF-KB in B group was markedly increased at 1 PBH [ (20. 3+/-3. 4) x 104A ] ,which was obviously higher than that in C group (2. 2 +/- 0. 4) x 104A , P <0.01]. It peaked at 3 PBH, and was still evidently higher than that in C group at 24 PBH ( P <0. 01). The expression of TNF-alpha mRNA was obviously higher than that in C group at 3 PBH ( P < 0. 01) , peaking at 6 PBH, and it was mainly expressed in myocardium. The expression of LVSP and +/- dp/dt max were lower, but LVEDP was higher than that in C group during 3 -24 PBH ( P <0.01).</p>

Experimental study on the relationship between neuropeptide substance P and wound healing in scalded rats / 中华烧伤杂志

<p><b>OBJECTIVE</b>To explore the relationship between neuropeptide substance P (SP) and wound healing in scalded rats.</p><p><b>METHODS</b>(1) Scalded rats with different degrees of scald injury were employed as the experimental model and were sacrificed at 24 post scald hour (PSH), and on 3, 7 and 14 post scald days (PSD). The SP content in the wound was detected with radioimmunoassay method. (2) The murine granulation tissue fibroblasts (GTF) were cultured with different culture media, and divided into control, SP and Spantide (SP receptor antagonism) groups. The effects of SP and Spantide on the cellular activity and apoptotic rate of murine GTF were assessed in vitro.</p><p><b>RESULTS</b>There was significant difference of the SP content among the superficial (145 +/- 78) ng/g, partial (94 +/- 48 ng/g) and full thickness (53 +/- 27 ng/g) scald wounds at 24 PSH (P < 0.01), while the SP content in partial thickness burn wound on 3 and 7 PSD obviously increased; and that in deep partial thickness burn wound obviously increased on 7 and 14 PSD. But the SP content remained unchanged in full thickness scald wound. (2) SP could promote the activity of GTF and inhibit its apoptosis (The GTF activity in control, SP groups were 0.21 +/- 0.05, 0.36 +/- 0.07, respectively, P < 0.01). Spantide could inhibit the interaction between SP and GTF.</p><p><b>CONCLUSION</b>SP can promote GTF proliferation, and the SP content in wound is closely associated with the depth of the injury and wound healing capacity.</p>

Influence of nuclear factor-kappaB activation on the expression of cytokines in monocytes stimulated by burn serum / 中华烧伤杂志

<p><b>OBJECTIVE</b>To investigate the effects of NF-kappaB activation on the expression of cytokines in monocytes stimulated by burn serum, so as to explore the mechanism in monocyte activation by burn serum.</p><p><b>METHODS</b>Peripheral blood monocytes (PBMCs) isolated from healthy volunteers were employed as the target cells. The cells were stimulated by serum from healthy volunteers (control), by serum from burn patients (burn serum), and by burn serum with addition of PDTC (pyrrolidine dithiocarbamate). Activation of monocytic NF-kappaB before stimulation and at 0.5, 1.0, 2.0 and 4.0 poststimulation hours (PSH) was assessed with electrophoretic mobility shift assay (EMSA). Expression of TNF-alpha and IL-8 mRNA at 1.0, 2.0, 4.0, 6.0 PSHs was assayed with in situ hybridization (ISH). Meanwhile, the contents of TNF-alpha and IL-8 in the supernatants were assayed by enzyme linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>The monocytic NF-kappaB activity in burn serum group increased significantly and reached the peak level at 1 PSH [(30.2 +/- 3.5) x 10(4) integration gray scale value] after the PBMCs were stimulated by burn serum, and it was obviously higher than that in control group [(4.4 +/- 0.8) x 10(4) integration gray scale value], (P < 0.01). It gradually decreased and returned to the pre-stimulation state at 2 PSH. The monocytic NF-kappaB activity in PDTC group decreased to [(6.8 +/- 0.9) x 10(4) integration gray scale value at 1 PSH] after the stimulation. The expression of TNF-alpha mRNA of the monocytes and the TNF-alpha level in the supernatant of the cultured PBMCs reached peak level at 1 PSHs after being stimulated by burn serum, and they were obviously higher than those in control group (P < 0.01). While the expression of IL-8 mRNA and the IL-8 level in the supernatant of the cultured PBMCs reached peak level at the 4 PSHs after being stimulated by burn serum, which were obviously higher than those in control group (P < 0.01) too. In addition, the synthesis and release of TNF-alpha (peaked at 1 PSH: 0.52 +/- 0.06 microg/L) and IL-8 (peaked at 4 PSH: 239 +/- 20 ng/L) in the supernatant of PBMCs in PDTC group were evidently higher than those in control group [(0.13 +/- 0.07) microg/L, < 156 ng/L] (P < 0.01).</p><p><b>CONCLUSION</b>Burn serum can induce the activation of NF-kappaB which lead to the synthesis and release of cytokines from PBMC. This result indicates that the activation of NF-kappaB plays important role in the secretion of cytokines from PBMCs induced by the burn serum.</p>

Effect of burn serum on the nuclear translocation of monocytic NF-kappaB p50/p65 / 中华烧伤杂志

<p><b>OBJECTIVE</b>To investigate the effects of burn serum on nuclear translocation of monocytic NF-kappaB heterodimers p50/p65 and the degradation of inhibiting kappaB (IkappaBalpha), so as to further explore the role of burn serum on the activation of monocytes.</p><p><b>METHODS</b>Peripheral blood monocytes (PBMCs) isolated from healthy volunteers were employed as the target cells. The cells were stimulated by the serum from healthy volunteers and burn patients, and by burn serum together with pyrrolidine dithiocarbamate (PDTC). Sera from normal healthy volunteers were taken as control. The nuclear translocation of monocytic p50 and p65 at 30th, 60th, 120th and 480th post stimulation minutes (PSM) was observed with laser confocal microscopy. The degradation of monocytic IkappaBalpha protein at 30th, 60th, 90th and 120th PSM was determined by Western blot.</p><p><b>RESULTS</b>Compared to that in control group, the nuclear translocation of monocytic p50 and p65 took place 30 min after the PBMCs were stimulated by burn serum, peaking at 30 to 60 min, but it gradually recovered to pre-stimulation state at 2 hrs with decreased intra-nuclear collection. Meanwhile, the IkappaBalpha degradation occurred within 30 min after PBMCs being stimulated by burn serum, and it peaked at 60 mins. However, IkappaBalpha gradually reappeared in the cytoplasm after 2 hrs of stimulation. PDTC (an antioxidants) could effectively inhibit monocytic IkappaBalpha degradation and nuclear translocation of NF-kappaB induced by burn serum.</p><p><b>CONCLUSION</b>Burn serum could induce nuclear translocation of p50 and p65 components of NF-kappaB in monocytes into the nucleus and degradation of IkappaBalpha, leading ultimately to the secretion of cytokines from the PBMCs.</p>

Effects of early postburn escharectomy on the permeability of cadiac and pulmonary blood vessels in rats / 第三军医大学学报

Objective　To evaluate the prophylactic-therapeutic role of escharectomy during early postburn stage on internal organ dysfunction. Methods　A total of 72 Wistar rats were randomized into 3 groups: burn without escharectomy group (A group), burn with escharectomy during early postburn stage (B group) and normal control. Vascular permeability were measured with 99Tc-labelled albumin. Furthermore, the effect of burn sera on permeability of monolayer of vascular endothelial cell (EC) was observed. Results　Vascular permeability in group B was significantly lower than in group A. When exposed to burn sera, EC monolayer permeability in group B is decreased when compared with group A. Conclusion　Escharectomy during early postburn stage could restore endothelial permeability

Effects of early postburn escharectomy on the permeability of cadiac and pulmonary blood vessels in rats / 第三军医大学学报

Objective　To evaluate the prophylactic-therapeutic role of escharectomy during early postburn stage on internal organ dysfunction. Methods　A total of 72 Wistar rats were randomized into 3 groups: burn without escharectomy group (A group), burn with escharectomy during early postburn stage (B group) and normal control. Vascular permeability were measured with 99Tc-labelled albumin. Furthermore, the effect of burn sera on permeability of monolayer of vascular endothelial cell (EC) was observed. Results　Vascular permeability in group B was significantly lower than in group A. When exposed to burn sera, EC monolayer permeability in group B is decreased when compared with group A. Conclusion　Escharectomy during early postburn stage could restore endothelial permeability